Recombinant human PNPO protein

PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Product Specifications

Product Name Alternative

Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.9 kDa (226aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PNPO, Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO, ATGP0801-10 µg, ATGP0801-20 µg, ATGP0801-50 µg, ATGP0801-100 µg, ATGP0801-250 µg, ATGP0801-500 µg, ATGP0801-1 mg, ATGP0801-10, ATGP0801-20, ATGP0801-50, ATGP0801-100, ATGP0801-250, ATGP0801-500, ATGP0801-1

References & Citations

Ngo EO., et al. (1998), Biochemistry., 37 (21) :7741-8; ; Kang JH., et al. (2004) . Eur J Biochem., 271 (12) :2452-61

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,0.1mM PMSF

Scientific Category

Metabolism

NCBI Accession Number

NP_060599

Uniprot Accession Number

Q9NVS9

Species

Human

AA Sequence

DPVKQFAAW FEEAVQCPDI GEANAMCLAT CTRDGKPSAR MLLLKGFGKD GFRFFTNFES RKGKELDSNP FASLVFYWEP LNRQVRVEGP VKKLPEEEAE CYFHSRPKSS QIGAVVSHQS SVIPDREYLR KKNEELEQLY QDQEVPKPKS WGGYVLYPQV MEFWQGQTNR LHDRIVFRRG LPTGDSPLGP MTHRGEEDWL YERLAP