Recombinant human PNPO protein
PNPO, also known as pyridoxine-5'-phosphate oxidase, is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6, or pyridoxal 5-prime-phosphate (PLP), is critical for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Recombinant human PNPO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography
Product Specifications
Product Name Alternative
Pyridoxine-5'-phosphate oxidase, Pyridoxine-5'-phosphate oxidase, FLJ10535, PDXPO
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.9 kDa (226aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Ngo EO., et al. (1998), Biochemistry., 37 (21) :7741-8; ; Kang JH., et al. (2004) . Eur J Biochem., 271 (12) :2452-61
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
NP_060599
Uniprot Accession Number
Q9NVS9
Species
Human
AA Sequence
Frequently Asked Questions
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