Recombinant human HDHD1A/PUDP protein

HDHD1A, also known as haloacid dehalogenase-like hydrolase domain-containing protein 1A, belongs to the adiponutrin family. This protein is of interest because it is an X-linked gene that escapes X-inactivation. It is particularly important in the understanding of human X chromosome structural organization as well as the mechanism of X-inactivation. Recombinant human HDHD1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Haloacid dehalogenase-like hydrolase domain-containing protein 1A, DXF68S1E, FAM16AX, GS1, pseudouridine 5'-phosphatase, HDHD1, Haloacid dehalogenase-like hydrolase domain-containing protein 1, Pseudouridine-5'-monophosphatase, 5'-PsiMPase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.4 kDa (248aa) confirmed by MALDI-TOF

Additionnal Information

Pseudouridine-5'-monophosphatase, Pseudouridine 5'-phosphatase, Pseudouridine 5' phosphatase, Pseudouridine 5' monophosphatase, HDHD1, Haloacid dehalogenase-like hydrolase domain-containing protein 1A, Haloacid dehalogenase-like hydrolase domain-containing protein 1, Haloacid dehalogenase like hydrolase domain containing protein 1A, Haloacid dehalogenase like hydrolase domain containing protein 1, GS1, FAM16AX, DXF68S1E, 5'-PsiMPase, 5' PsiMPase, ATGP0797-10 µg, ATGP0797-20 µg, ATGP0797-50 µg, ATGP0797-100 µg, ATGP0797-250 µg, ATGP0797-500 µg, ATGP0797-1 mg, ATGP0797-010, ATGP0797-020, ATGP0797-050, ATGP0797-100, ATGP0797-250, ATGP0797-500, ATGP0797-01M

References & Citations

Salido E.C., et al. (1992), Am. J. Hum. Genet., 50: 303-316.; ; Soehnge H., et al. (1997) . Gene., 185: 257-263

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_036212

Uniprot Accession Number

Q08623

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE