Recombinant human Spermidine synthase/SRM protein

SRM is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. This protein is one of four enzymes in the polyamine-biosynthetic pathway and carries out the final step of spermidine biosynthesis. Recombinant human SRM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Spermidine synthase, PAPT, SPDSY, SPS1, SRML1, Putrescine aminopropyltransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.9 kDa (322aa) confirmed by MALDI-TOF

Additionnal Information

SRM, Spermidine synthase, Spermidine synthase, PAPT, SPDSY, SPS1, SRML1, ATGP0794-10 µg, ATGP0794-20 µg, ATGP0794-50 µg, ATGP0794-100 µg, ATGP0794-250 µg, ATGP0794-500 µg, ATGP0794-1 mg, ATGP0794-10, ATGP0794-20, ATGP0794-50, ATGP0794-100, ATGP0794-250, ATGP0794-500, ATGP0794-1

References & Citations

Wu H., et al. (2007) Biochemistry. 46 (28) :8331-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_003123

Uniprot Accession Number

P19623

Species

Human

AA Sequence

MEPGPDGPAA SGPAAIREGW FRETCSLWPG QALSLQVEQL LHHRRSRYQD ILVFRSKTYG NVLVLDGVIQ CTERDEFSYQ EMIANLPLCS HPNPRKVLII GGGDGGVLRE VVKHPSVESV VQCEIDEDVI QVSKKFLPGM AIGYSSSKLT LHVGDGFEFM KQNQDAFDVI ITDSSDPMGP AESLFKESYY QLMKTALKED GVLCCQGECQ WLHLDLIKEM RQFCQSLFPV VAYAYCTIPT YPSGQIGFML CSKNPSTNFQ EPVQPLTQQQ VAQMQLKYYN SDVHRAAFVL PEFARKALND VS