Recombinant human PBLD protein

PBLD, also known as MAWBP, is a phenazine biosynthesis-like protein (PhzF) family. Expressed in most tissues, PBLD is the only representative of the PhzF family in the human genome. It is participate in the MAPK signaling pathway. Involved in multiple basic cellular functions, expression of PBLD is elevated in several disease processes, including Insulin resistance, folate deficiency and hypotension. Recombinant human PBLD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phenazine biosynthesis-like domain-containing protein, FLJ14767, MAWBP, MAWDBP.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.9 kDa (308aa) confirmed by MALDI-TOF

Additionnal Information

PBLD, Phenazine biosynthesis-like domain-containing protein, FLJ14767, MAWBP, MAWDBP., ATGP0790-10 µg, ATGP0790-20 µg, ATGP0790-50 µg, ATGP0790-100 µg, ATGP0790-250 µg, ATGP0790-500 µg, ATGP0790-1 mg, ATGP0790-10, ATGP0790-20, ATGP0790-50, ATGP0790-100, ATGP0790-250, ATGP0790-500, ATGP0790-1

References & Citations

Mavrodi DV., et al. (2004) Acta Crystallogr D Biol Crystallogr. 60 (1) :184-6. ; ; Parsons JF., et al. (2004) Biochemistry. 43 (39) :12427-35.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_071412

Uniprot Accession Number

P30039

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MKLPIFIADA FTARAFRGNP AAVCLLENEL DEDMHQKIAR EMNLSETAFI RKLHPTDNFA QSSCFGLRWF TPASEVPLCG HATLASAAVL FHKIKNMNST LTFVTLSGEL RARRAEDGIV LDLPLYPAHP QDFHEVEDLI KTAIGNTLVQ DICYSPDTQK LLVRLSDVYN RSFLENLKVN TENLLQVENT GKVKGLILTL KGEPGGQTQA FDFYSRYFAP WVGVAEDPVT GSAHAVLSSY WSQHLGKKEM HAFQCSHRGG ELGISLRPDG RVDIRGGAAV VLEGTLTA