Recombinant human Purine nucleoside phosphorylase/PNP protein

PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Product Name Alternative

Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PuNP

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 120unit/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Molecular Weight

34.2 kDa (309aa) confirmed by MALDI-TOF

Additionnal Information

Purine nucleoside phosphorylase, PNP, ATGP0786-10 µg, ATGP0786-20 µg, ATGP0786-50 µg, ATGP0786-100 µg, ATGP0786-250 µg, ATGP0786-500 µg, ATGP0786-1 mg, ATGP0786-10, ATGP0786-20, ATGP0786-50, ATGP0786-100, ATGP0786-250, ATGP0786-500, ATGP0786-1

References & Citations

Dautant A., et al. (2010) J Biol Chem. 285 (38) :29502-10.; ; Balakrishnan K., et al. (2010) Blood. 116 (6) :886-92.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_000261

Uniprot Accession Number

P00491

Species

Human

AA Sequence

MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS