Recombinant human NANS protein
NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins) -like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Sialic acid synthase, SAS, N acetylneuraminate synthase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
42.4 kDa (379aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Milstein O., et al. (2008) J Biol Chem. 283 (49) :34414-22.; ; Aldape MJ., et al. (2007) J Infect Dis. 195 (12) :1838-45.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_061819
Uniprot Accession Number
Q9NR45
Species
Human
AA Sequence
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