Recombinant human PMM1 protein
PMM1 (Phosphomannomutase 1) is an enzyme that involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. This enzyme catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. In addition, it may be responsible for the degradation of glucose-1, 6-bisphosphate in ischemic brain. Recombinant human PMM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phosphomannomutase 1, Sec53, PMMH22
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31.9 kDa (282aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Veiga-da-Cunha M., et al. (2008) J Biol Chem. 283 (49) :33988-93.; ; Silvaggi N.R., et al. (2006) J. Biol. Chem. 281:14918-14926;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl, 0.1mM PMSF
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002667
Uniprot Accession Number
Q92871
Species
Human
AA Sequence
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