Recombinant human CBR4 protein

CBR4 belongs to the short-chain dehydrogenase/reductase family. The formation of heteroteramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones. It plays a role in biosynthesis of fatty acids in mitochondria and has broad substrate specificity and reduces 9, 10-phenanthrenequinone, 1, 4-benzoquinone and various other o-quinones and p-quinones (in vitro) . Recombinant human CBR4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Carbonyl reductase family member 4, Carbonyl reductase family member 4, Quinone reductase CBR4, 3-oxoacyl-[acyl-carrier-protein] reductase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27.5 kDa (257aa) confirmed by MALDI-TOF

Additionnal Information

CBR4, Carbonyl reductase family member 4, Carbonyl reductase family member 4, Quinone reductase CBR4, 3-oxoacyl-[acyl-carrier-protein] reductase, ATGP0781-10 µg, ATGP0781-20 µg, ATGP0781-50 µg, ATGP0781-100 µg, ATGP0781-250 µg, ATGP0781-500 µg, ATGP0781-1 mg, ATGP0781-10, ATGP0781-20, ATGP0781-50, ATGP0781-100, ATGP0781-250, ATGP0781-500, ATGP0781-1

References & Citations

Endo S., et al. (2008) Biochem Biophys Res Commun. 26; ;377 (4) :1326-30; ; Chen Z., et al. (2009) FASEB J. 23:3682-3691;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 5mM DTT, 200 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

AAH21973.1

Uniprot Accession Number

Q8N4T8

Species

Human

AA Sequence

MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL