Recombinant human TNIP1 protein

TNIP1 (TNFAIP3-interacting protein 1) has been shown to interacts with zinc finger protein A20/TNFAIP3 and inhibits TNF-induced NF-kappa-B-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal. In addition, interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. It may regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. Recombinant human TNIP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TNFAIP3 interacting protein 1, ABIN-1, NAF1, VAN, Nip40-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

51.8 kDa (458aa) confirmed by MALDI-TOF

Additionnal Information

TNIP1, TNFAIP3 interacting protein 1, ABIN-1, NAF1, VAN, Nip40-1, ATGP0780-10 µg, ATGP0780-20 µg, ATGP0780-50 µg, ATGP0780-100 µg, ATGP0780-250 µg, ATGP0780-500 µg, ATGP0780-1 mg, ATGP0780-10, ATGP0780-20, ATGP0780-50, ATGP0780-100, ATGP0780-250, ATGP0780-500, ATGP0780-1

References & Citations

He CF., et al. (2010) Lupus 19 (10) :1181-6; ; Gateva V., et al. (2009) Nat Genet.41 (11) :1228-33.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 5mM DTT, 200 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006049

Uniprot Accession Number

Q15025

Species

Human

AA Sequence

MSNVTASPTA PACPSDKPAP VQKPPSSGTS SEFEVVTPEE QNSPESSSHA NAMALGPLPR EDGNLMLHLQ RLETTLSVCA EEPDHGQLFT HLGRMALEFN RLASKVHKNE QRTSILQTLC EQLRKENEAL KAKLDKGLEQ RDQAAERLRE ENLELKKLLM SNGNKEGASG RPGSPKMEGT GKKAVAGQQQ ASVTAGKVPE VVALGAAEKK VKMLEQQRSE LLEVNKQWDQ HFRSMKQQYE QKITELRQKL ADLQKQVTDL EAEREQKQRD FDRKLLLAKS KIEMEETDKE QLTAEAKELR QKVKYLQDQL SPLTRQREYQ EKEIQRLNKA LEEALSIQTP PSSPPTAFGS PEGAGALLRK QELVTQNELL KQQVKIFEED FQRERSDRER MNEEKEELKK QVEKLQAQVT LSNAQLKAFK DEEKAREALR QQKRKAKA