Recombinant human Pyridoxal Kinase/PDXK protein
PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recombinant human PDXK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
37.6 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Merrill AH Jr., et al. (1984) J Nutr. 114 (9) :1664-74.; ; Hanna M C., et al. (1997) J Biol Chem. 272 (16) :10756-60.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_003672
Uniprot Accession Number
O00764
Species
Human
AA Sequence
Frequently Asked Questions
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