Recombinant human Pyridoxal Kinase/PDXK protein

PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recombinant human PDXK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.6 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

Pyridoxal Kinase, PDXK, Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase, ATGP0777-10 µg, ATGP0777-20 µg, ATGP0777-50 µg, ATGP0777-100 µg, ATGP0777-250 µg, ATGP0777-500 µg, ATGP0777-1 mg, ATGP0777-10, ATGP0777-20, ATGP0777-50, ATGP0777-100, ATGP0777-250, ATGP0777-500, ATGP0777-1

References & Citations

Merrill AH Jr., et al. (1984) J Nutr. 114 (9) :1664-74.; ; Hanna M C., et al. (1997) J Biol Chem. 272 (16) :10756-60.;

Other References

Maity AN, et al. The Nitrogen Atom of Vitamin B6 Is Essential for the Catalysis of Radical Aminomutases. (Int J Mol Sci. 2022) {https://pubmed.ncbi.nlm.nih.gov/35563602/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_003672

Uniprot Accession Number

O00764

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAEL LSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL