Recombinant human Glutathione synthetase/GSS protein

Glutathione synthetase, also known GSS, is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) ; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system. Recombinant human GSS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glutathione synthetase, GSHS, GSH synthetase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

54.5 kDa (494aa) confirmed by MALDI-TOF

Additionnal Information

GSS, Glutathione synthetase, GSHS, GSH synthetase, ATGP0776-10 µg, ATGP0776-20 µg, ATGP0776-50 µg, ATGP0776-100 µg, ATGP0776-250 µg, ATGP0776-500 µg, ATGP0776-1 mg, ATGP0776-10, ATGP0776-20, ATGP0776-50, ATGP0776-100, ATGP0776-250, ATGP0776-500, ATGP0776-1

References & Citations

Polekhina G., et al. (1999) EMBO J. 18:3204-3213.; ; Huanq Z A., et al. (2000) Biochim Biophys Acta. 1493:48-55.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_000169

Uniprot Accession Number

P48637

Species

Human

AA Sequence

MATNWGSLLQ DKQQLEELAR QAVDRALAEG VLLRTSQEPT SSEVVSYAPF TLFPSLVPSA LLEQAYAVQM DFNLLVDAVS QNAAFLEQTL SSTIKQDDFT ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF QRSADGSPAL KQIEINTISA SFGGLASRTP AVHRHVLSVL SKTKEAGKIL SNNPSKGLAL GIAKAWELYG SPNALVLLIA QEKERNIFDQRAIENELLAR NIHVIRRTFE DISEKGSLDQ DRRLFVDGQE IAVVYFRDGY MPRQYSLQNW EARLLLERSH AAKCPDIATQ LAGTKKVQQE LSRPGMLEML LPGQPEAVAR LRATFAGLYS LDVGEEGDQA IAEALAAPSR FVLKPQREGG GNNLYGEEMV QALKQLKDSE ERASYILMEK IEPEPFENCL LRPGSPARVV QCISELGIFG VYVRQEKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV