Recombinant human RhoGDI 1/ARHGDIA protein

Rho GDP-dissociation inhibitor 1, also known ARHGDIA, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. Localized to the cytoplasm, ARHGDIA inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans ARHGDIA can be phosphorylated at Ser 101 by p21-activated kinase, an event that inhibits ARHGDIA activity and may result in positive feedback regulation of certain ARHGDIA target proteins. Recombinant human ARHGDIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rho GDP-dissociation inhibitor 1, GDIA1, RHOGDI, RHOGDI-1, Rho-GDI alpha

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.9 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

ARHGDIA, Rho GDP-dissociation inhibitor 1, GDIA1, RHOGDI, RHOGDI-1, Rho-GDI alpha, ATGP0775-10 µg, ATGP0775-20 µg, ATGP0775-50 µg, ATGP0775-100 µg, ATGP0775-250 µg, ATGP0775-500 µg, ATGP0775-1 mg, ATGP0775-10, ATGP0775-20, ATGP0775-50, ATGP0775-100, ATGP0775-250, ATGP0775-500, ATGP0775-1

References & Citations

DerMardirossian C., et al. (2006) Mol Biol Cell. 17:4760-4768.; ; Leffers H., et al. (1993) Exp Cell Res. 209:165-174.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004300

Uniprot Accession Number

P52565

Species

Human

AA Sequence

MSVNYKPPAQ KSIQEIQELD KDDESLRKYK EALLGRVAVS ADPNVPNVVV TGLTLVCSSA PGPLELDLTG DLESFKKQSF VLKEGVEYRI KISFRVNREI VSGMKYIQHT YRKGVKIDKT DYMVGSYGPR AEEYEFLTPV EEAPKGMLAR GSYSIKSRFT DDDKTDHLSW EWNLTIKKDW KD