Recombinant human RhoGDI 1/ARHGDIA protein
Rho GDP-dissociation inhibitor 1, also known ARHGDIA, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. Localized to the cytoplasm, ARHGDIA inhibits the dissociation of GDP from Rho proteins, thereby preventing GTP from binding to and subsequently activating Rho proteins. In humans ARHGDIA can be phosphorylated at Ser 101 by p21-activated kinase, an event that inhibits ARHGDIA activity and may result in positive feedback regulation of certain ARHGDIA target proteins. Recombinant human ARHGDIA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Rho GDP-dissociation inhibitor 1, GDIA1, RHOGDI, RHOGDI-1, Rho-GDI alpha
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.9 kDa (202aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
DerMardirossian C., et al. (2006) Mol Biol Cell. 17:4760-4768.; ; Leffers H., et al. (1993) Exp Cell Res. 209:165-174.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004300
Uniprot Accession Number
P52565
Species
Human
AA Sequence
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