Recombinant human AK1 protein

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Product Name Alternative

Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity: > 600unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Molecular Weight

23.7 kDa (214aa) confirmed by MALDI-TOF

Additionnal Information

AK1, Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, ATGP0769-10 µg, ATGP0769-20 µg, ATGP0769-50 µg, ATGP0769-100 µg, ATGP0769-250 µg, ATGP0769-500 µg, ATGP0769-1 mg, ATGP0769-10, ATGP0769-20, ATGP0769-50, ATGP0769-100, ATGP0769-250, ATGP0769-500, ATGP0769-1

References & Citations

Terzic A., et al. (2007) J Biol Chem. 282 (43) :31366-72.; ; Morelli A., et al (2007) Curr Eye Res. 32 (3) :249-57.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_000467

Uniprot Accession Number

P00568

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK