Recombinant human Crk protein
CRK is a member of signaling adapter protein family that binds to several tyrosine-phosphorylated proteins. This protein is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an SH2 followed by two SH3 domains (src-homology domains) . The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Recombinant human CRK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Adapter molecule crk, Proto-oncogene c-Crk, p38, CRKII
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
25.0 kDa (224aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Muir TW., et al (2006) Biochemistry. 45 (29) :8874-84.; ; Inagaki F., et al. (2007) Nat Struct Mol Biol. 14 (6) :503-10.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_005197
Uniprot Accession Number
P46108
Species
Human
AA Sequence
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