Recombinant human Bleomycin Hydrolase/BLMH protein
BLMH is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug BLM. It catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant human BLMH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Bleomycin hydrolase, BH, BLM hydrolase, BMH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
54.7 kDa (475aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lazo JS., et al. (2007) Neuroscience. 146 (3) :890-900; ; Abernethy J., et al (1991) Proc Natl Acad Sci u S A. 96 (8) :4680-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_000377
Uniprot Accession Number
Q13867
Species
Human
AA Sequence
Frequently Asked Questions
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