Recombinant human SBDS protein

Ribosome maturation protein SBDS is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. It may be involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Shwachman-Diamond syndrome is a rare autosomal recessive disorder caused by mutations in the SBDS gene. Recombinant human SBDS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ribosome maturation protein SBDS, CGI-97, SDS, SWDS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.9 kDa (270aa) confirmed by MALDI-TOF

Additionnal Information

SBDS, Ribosome maturation protein SBDS, CGI-97, SDS, SWDS, ATGP0762-10 µg, ATGP0762-20 µg, ATGP0762-50 µg, ATGP0762-100 µg, ATGP0762-250 µg, ATGP0762-500 µg, ATGP0762-1 mg, ATGP0762-10, ATGP0762-20, ATGP0762-50, ATGP0762-100, ATGP0762-250, ATGP0762-500, ATGP0762-1

References & Citations

Tooviainen-Salo S., et al (2010) Duodecim. 126 (14) :1711-9.; ; Hesling C., et al (2007) Exp. Cell Res. 313:4180-4195

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 50 mM NaCl, 0.1mM EDTA

Scientific Category

Metabolism

NCBI Accession Number

NP_057122

Uniprot Accession Number

Q9Y3A5

Species

Human

AA Sequence

MSIFTPTNQI RLTNVAVVRM KRAGKRFEIA CYKNKVVGWR SGVEKDLDEV LQTHSVFVNV SKGQVAKKED LISAFGTDDQ TEICKQILTK GEVQVSDKER HTQLEQMFRD IATIVADKCV NPETKRPYTV ILIERAMKDI HYSVKTNKST KQQALEVIKQ LKEKMKIERA HMRLRFILPV NEGKKLKEKL KPLIKVIESE DYGQQLEIVC LIDPGCFREI DELIKKETKG KGSLEVLNLK DVEEGDEKFE