Recombinant human PGLS protein
6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
6-phosphogluconolactonase, 6PGL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
29.7 kDa (278aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Collard F., et al. (1999) FEBS Lett. 459 (2) :223-6. ; ; Hong M K., et al. (2008) Acta Crystallogr Sect F Struct Biol Cryst Commun. 64 (11) :1016-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_036220
Uniprot Accession Number
O95336
Species
Human
AA Sequence
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