Recombinant human PGLS protein

6-phosphogluconolactonase, also known PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

6-phosphogluconolactonase, 6PGL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.7 kDa (278aa) confirmed by MALDI-TOF

Additionnal Information

PGLS, 6-phosphogluconolactonase, 6PGL, ATGP0758-10 µg, ATGP0758-20 µg, ATGP0758-50 µg, ATGP0758-100 µg, ATGP0758-250 µg, ATGP0758-500 µg, ATGP0758-1 mg, ATGP0758-10, ATGP0758-20, ATGP0758-50, ATGP0758-100, ATGP0758-250, ATGP0758-500, ATGP0758-1

References & Citations

Collard F., et al. (1999) FEBS Lett. 459 (2) :223-6. ; ; Hong M K., et al. (2008) Acta Crystallogr Sect F Struct Biol Cryst Commun. 64 (11) :1016-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_036220

Uniprot Accession Number

O95336

Species

Human

AA Sequence

MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL