Recombinant human MAGEA3 protein

Melanoma-associated antigen 3, also known MAGEA3, is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen widely expressed in solid and hematologic malignancies, but not in normal tissues, with the exception of testis and placenta. Therefore, MAGEA3 is an excellent candidate tumor antigen. Recombinant human MAGEA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Melanoma-associated antigen 3, CT1.3, HIP8, HYPD, MAGE3, MZ2 D

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.1 kDa (337aa) confirmed by MALDI-TOF

Additionnal Information

MAGEA3, Melanoma-associated antigen 3, CT1.3, HIP8, HYPD, MAGE3, MZ2 D, ATGP0757-10 µg, ATGP0757-20 µg, ATGP0757-50 µg, ATGP0757-100 µg, ATGP0757-250 µg, ATGP0757-500 µg, ATGP0757-1 mg, ATGP0757-10, ATGP0757-20, ATGP0757-50, ATGP0757-100, ATGP0757-250, ATGP0757-500, ATGP0757-1

References & Citations

Roqner u C., et al. (1995) Genomics. 29 (3) :725-31.; ; Jang S J., et al. (2001) Cancer Res. 61 (21) :7959-63.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_005353.1

Uniprot Accession Number

P43357

Species

Human

AA Sequence

PLEQRS QHCKPEEGLE ARGEALGLVG AQAPATEEQE AASSSSTLVE VTLGEVPAAE SPDPPQSPQG ASSLPTTMNY PLWSQSYEDS SNQEEEGPST FPDLESEFQA ALSRKVAELV HFLLLKYRAR EPVTKAEMLG SVVGNWQYFF PVIFSKASSS LQLVFGIELM EVDPIGHLYI FATCLGLSYD GLLGDNQIMP KAGLLIIVLA IIAREGDCAP EEKIWEELSV LEVFEGREDS ILGDPKKLLT QHFVQENYLE YRQVPGSDPA CYEFLWGPRA LVETSYVKVL HHMVKISGGP HISYPPLHEW VLREGEE