Recombinant human LIF protein
Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Recombinant human LIF protein, fused to His GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Leukemia inhibitory factor, CDF, HILDA, DIA, D FACTOR, MLPLI, Emfilermin
Expression System
E.coli
Antigen Species
Human
Tag
His-GST-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.2 kDa (415aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gillett N A., et al. (1993) Growth Factors. 9:301-305.; ; Stahl N., et al. (1994) J Neurobiol. 25:1454-1466.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl
Scientific Category
Growth factors
NCBI Accession Number
NP_002300
Uniprot Accession Number
P15018
Species
Human
AA Sequence
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