Recombinant human LIF protein

Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Recombinant human LIF protein, fused to His GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Leukemia inhibitory factor, CDF, HILDA, DIA, D FACTOR, MLPLI, Emfilermin

Expression System

E.coli

Antigen Species

Human

Tag

His-GST-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.2 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

LIF, Leukemia inhibitory factor, CDF, HILDA, DIA, D FACTOR, MLPLI, Emfilermin, ATGP0756-10 µg, ATGP0756-20 µg, ATGP0756-50 µg, ATGP0756-100 µg, ATGP0756-250 µg, ATGP0756-500 µg, ATGP0756-1 mg, ATGP0756-10, ATGP0756-20, ATGP0756-50, ATGP0756-100, ATGP0756-250, ATGP0756-500, ATGP0756-1

References & Citations

Gillett N A., et al. (1993) Growth Factors. 9:301-305.; ; Stahl N., et al. (1994) J Neurobiol. 25:1454-1466.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Scientific Category

Growth factors

NCBI Accession Number

NP_002300

Uniprot Accession Number

P15018

Species

Human

AA Sequence

MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF