Recombinant human HPD protein

4-hydroxyphenylpyruvate dioxygenase isoform 1, also known as HPD, is an Fe-containing enzyme, that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, HPD uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding HPD are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant human HPD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

4-hydroxyphenylpyruvate dioxygenase isoform 1, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD, 4-hydroxyphenylpyruvic acid oxidase, Glyoxalase domain containing 3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47 kDa (413aa) confirmed by MALDI-TOF

Additionnal Information

HPD, 4-hydroxyphenylpyruvate dioxygenase isoform 1, 4-HPPD, 4HPPD, GLOD3, HPPDASE, PPD, ATGP0755-10 µg, ATGP0755-20 µg, ATGP0755-50 µg, ATGP0755-100 µg, ATGP0755-250 µg, ATGP0755-500 µg, ATGP0755-1 mg, ATGP0755-10, ATGP0755-20, ATGP0755-50, ATGP0755-100, ATGP0755-250, ATGP0755-500, ATGP0755-1

References & Citations

Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; uetschi u., et al. (1997) Genomics. 44:292-299.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_002141

Uniprot Accession Number

P32754

Species

Human

AA Sequence

MTTYSDKGAK PERGRFLHFH SVTFWVGNAK QAASFYCSKM GFEPLAYRGL ETGSREVVSH VIKQGKIVFV LSSALNPWNK EMGDHLVKHG DGVKDIAFEV EDCDYIVQKA RERGAKIMRE PWVEQDKFGK VKFAVLQTYG DTTHTLVEKM NYIGQFLPGY EAPAFMDPLL PKLPKCSLEM IDHIVGNQPD QEMVSASEWY LKNLQFHRFW SVDDTQVHTE YSSLRSIVVA NYEESIKMPI NEPAPGKKKS QIQEYVDYNG GAGVQHIALK TEDIITAIRH LRERGLEFLS VPSTYYKQLR EKLKTAKIKV KENIDALEEL KILVDYDEKG YLLQIFTKPV QDRPTLFLEV IQRHNHQGFG AGNFNSLFKA FEEEQNLRGN LTNMETNGVV PGM