Recombinant human PA28 alpha/PSME1 protein

PSME1 is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring. Recombinant human PSME1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome activator subunit 1, 11S regulator complex subunit alpha, REG-alpha, Activator of multicatalytic protease subunit 1, Interferon gamma up-regulated I-5111 protein, IGUP I-5111, Proteasome activator 28 subunit alpha, PA28a, PA28alpha, IFI5111

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30.8 kDa (269aa) confirmed by MALDI-TOF

Additionnal Information

PA28 Activator a Subunit, PSME1, Proteasome activator complex subunit 1, IFI5111, MGC8628, PA28A, PA28alpha, REGalpha, ATGP0749-10 µg, ATGP0749-20 µg, ATGP0749-50 µg, ATGP0749-100 µg, ATGP0749-250 µg, ATGP0749-500 µg, ATGP0749-1 mg, ATGP0749-10, ATGP0749-20, ATGP0749-50, ATGP0749-100, ATGP0749-250, ATGP0749-500, ATGP0749-1

References & Citations

Ahn K.. et al. (1996) J Biol Chem. 271 (30) :18237-42; ; Murata S., et al. (2001) EMBO J.. 20 (21) :5898-907.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_006254

Uniprot Accession Number

Q06323

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY