Recombinant human PA28 alpha/PSME1 protein
PSME1 is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring. Recombinant human PSME1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
30.8 kDa (269aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ahn K.. et al. (1996) J Biol Chem. 271 (30) :18237-42; ; Murata S., et al. (2001) EMBO J.. 20 (21) :5898-907.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_006254
Uniprot Accession Number
Q06323
Species
Human
AA Sequence
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