Recombinant human PPCDC protein

PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B) . The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.6 kDa (224aa) confirmed by MALDI-TOF

Additionnal Information

PPCDC, Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585, ATGP0748-10 µg, ATGP0748-20 µg, ATGP0748-50 µg, ATGP0748-100 µg, ATGP0748-250 µg, ATGP0748-500 µg, ATGP0748-1 mg, ATGP0748-10, ATGP0748-20, ATGP0748-50, ATGP0748-100, ATGP0748-250, ATGP0748-500, ATGP0748-1

References & Citations

Strauss E., et al. (2004) Biochemistry. 43 (49) :15520-33. ; ; Daugherty M., et al. (2002) J Biol Chem. 277 (24) :21431-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Metabolism

NCBI Accession Number

AAH14409

Uniprot Accession Number

Q96CD2

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS