Recombinant human PPCDC protein
PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B) . The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
24.6 kDa (224aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Strauss E., et al. (2004) Biochemistry. 43 (49) :15520-33. ; ; Daugherty M., et al. (2002) J Biol Chem. 277 (24) :21431-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Metabolism
NCBI Accession Number
AAH14409
Uniprot Accession Number
Q96CD2
Species
Human
AA Sequence
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