Recombinant human MAPRE3 protein

MAPRE1 is a microtubule associated protein that interacts with the colorectal adenomatous polyposis coli tumor suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability. This protein is related to MAPRE1 and likewise associates with the microtubule cytoskeleton. MAPRE3 is expressed predominantly in the central nervous system and preferentially associates with APCL, a homolog of the adenomatous polyposis coli tumor suppressor protein. Recombinant human MAPRE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Microtubule-associated protein RP/EB family member 3, EB3, EBF3, EBF3-S, RP3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.1 kDa (301aa) ) confirmed by MALDI-TOF

Additionnal Information

MAPRE3, microtubule-associated protein RP/EB family member 3, EB3, EBF3, EBF3-S, RP3, ATGP0746-10 µg, ATGP0746-20 µg, ATGP0746-50 µg, ATGP0746-100 µg, ATGP0746-250 µg, ATGP0746-500 µg, ATGP0746-1 mg, ATGP0746-10, ATGP0746-20, ATGP0746-50, ATGP0746-100, ATGP0746-250, ATGP0746-500, ATGP0746-1

References & Citations

Berrueta L., et al. (1998) Proc Natl Acad Sci u S A. 95 (18) :10596-601. ; ; Bu W., et al. (2003) J Biol Chem. 278 (50) :49721-31.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_036458

Uniprot Accession Number

Q9UPY8

Species

Human

AA Sequence

MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y