Recombinant human MAPRE3 protein
MAPRE1 is a microtubule associated protein that interacts with the colorectal adenomatous polyposis coli tumor suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability. This protein is related to MAPRE1 and likewise associates with the microtubule cytoskeleton. MAPRE3 is expressed predominantly in the central nervous system and preferentially associates with APCL, a homolog of the adenomatous polyposis coli tumor suppressor protein. Recombinant human MAPRE3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Microtubule-associated protein RP/EB family member 3, EB3, EBF3, EBF3-S, RP3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.1 kDa (301aa) ) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Berrueta L., et al. (1998) Proc Natl Acad Sci u S A. 95 (18) :10596-601. ; ; Bu W., et al. (2003) J Biol Chem. 278 (50) :49721-31.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_036458
Uniprot Accession Number
Q9UPY8
Species
Human
AA Sequence
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