Recombinant human GRPEL1 protein
GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
GrpE protein homolog 1 mitochondrial, FLJ25609, HMGE
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.6 kDa (211aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Borges JC., et al. (2003) J Biol Chem. 278 (37) :35337-44.; ; Hohfeld J., et al. (1997) EMBO J. 16 (20) :6209-16.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_079472
Uniprot Accession Number
Q9HAV7
Species
Human
AA Sequence
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