Recombinant human Retinol Binding Protein 5/RBP5 protein
RBP5 belongs to the Retinol-binding proteins family. Assessment of retinol-binding protein is used to determine visceral protein mass in nutritional studies related to health. RBP5 is higher expression in adult kidney and liver and to a lesser extent in adult and fetal spleen, adult lymph nodes and appendix, and fetal liver and kidney. It is strongly decreased in hepatocellular carcinoma tissues. Recombinant human RBP5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Retinol binding protein 5, CRBP-III, CRBP3, CRBPIII, Cellular retinol-binding protein III, Retinol binding protein 5, HRBPiso
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.1 kDa (155aa), confirmed by MALDI-TOF
Additionnal Information
References & Citations
Folli C., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:3710-3715; ; Ho J.C.Y., et al. (2007) J. Cancer Res. Clin. Oncol. 133:929-936;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Signal Transduction
NCBI Accession Number
NP_113679
Uniprot Accession Number
P82980
Species
Human
AA Sequence
MPPNLTGYYR FVSQKNMEDY LQALNISLAV RKIALLLKPD KEIEHQGNHM TVRTLSTFRN YTVQFDVGVE FEEDLRSVDG RKCQTIVTWE EEHLVCVQKG EVPNRGWRHW LEGEMLYLEL TARDAVCEQV FRKVR
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