Recombinant human NUDT1/MTH1 protein

NuDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. It is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. And this protein expressed at much higher levels in proliferating cells than in resting cells. Recombinant human NuDT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Oxidized purine nucleoside triphosphate hydrolase, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, Methylated purine nucleoside triphosphate hydrolase, Nucleoside diphosphate-linked moiety X motif 1 (Nudix motif 1)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.1 kDa (176aa) confirmed by MALDI-TOF

Additionnal Information

"7,8-dihydro-8-oxoguanine triphosphatase", 78-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21, MTH1, Nudix motif 1, NUDT1, ATGP0742-10 µg, ATGP0742-20 µg, ATGP0742-50 µg, ATGP0742-100 µg, ATGP0742-250 µg, ATGP0742-500 µg, ATGP0742-1 mg, ATGP0742-010, ATGP0742-020, ATGP0742-050, ATGP0742-100, ATGP0742-250, ATGP0742-500, ATGP0742-01M

References & Citations

Satoh J., et al (2000) Eur J Neurol. 7 (6) :673-7.; ; Oda H., et al. (1997) J. Biol. Chem. 272:17843-17850;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_945191

Uniprot Accession Number

P36639

Species

Human

AA Sequence

MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV