Recombinant human HDHD4/NANP protein

NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating N-acylneuraminate 9-phosphate to form N-acylneuraminate (N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate) . Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium. Recombinant human NANP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, N-acetylneuraminic acid phosphatase, HDHD4, Haloacid dehalogenase-like hydrolase domain containing 4, C20orf147

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.9 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

NANP, N-acylneuraminate-9-phosphatase, HDHD4, C20orf147, Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase, ATGP0741-10 µg, ATGP0741-20 µg, ATGP0741-50 µg, ATGP0741-100 µg, ATGP0741-250 µg, ATGP0741-500 µg, ATGP0741-1 mg, ATGP0741-10, ATGP0741-20, ATGP0741-50, ATGP0741-100, ATGP0741-250, ATGP0741-500, ATGP0741-1

References & Citations

Hao J., et al. (2006) Biochem J. 397: 195-201.; ; Maliekal P., et al. (2006) Glycobiology. 16:165-172;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_689880

Uniprot Accession Number

Q8TBE9

Species

Human

AA Sequence

MGLS RVRAVFFDLD NTLIDTAGAS RRGMLEVIKL LQSKYHYKEE AEIICDKVQV KLSKECFHPY NTCITDLRTS HWEEAIQETK GGAANRKLAE ECYFLWKSTR LQHMTLAEDV KAMLTELRKE VRLLLLTNGD RQTQREKIEA CACQSYFDAV VVGGEQREEK PAPSIFYYCC NLLGVQPGDC VMVGDTLETD IQGGLNAGLK ATVWINKNGI VPLKSSPVPH YMVSSVLELP ALLQSIDCKV SMST