Recombinant human AA protein

AASDHPPT belongs to the P-Pant transferase superfamily. This protein catalyzes the post-translational modification of target proteins by phosphopantetheine and can transfer the 4-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN (in vitro) . It is detected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney. Recombinant human AASDHPPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, CGI-80, LYS2, LYS5, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

36.4 kDa (316aa) confirmed by MALDI-TOF

Additionnal Information

AA, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, AASD-PPT, CGI-80, LYS2, LYS5, L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, ATGP0740-10 µg, ATGP0740-20 µg, ATGP0740-50 µg, ATGP0740-100 µg, ATGP0740-250 µg, ATGP0740-500 µg, ATGP0740-1 mg, ATGP0740-10, ATGP0740-20, ATGP0740-50, ATGP0740-100, ATGP0740-250, ATGP0740-500, ATGP0740-1

References & Citations

Krupenko SA., et al. (2010) J Biol Chem. 285 (3) :1627-33.; ; Geraghty MT., et al. (2001) Mol Genet Metab. 72 (4) :336-42.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_056238

Uniprot Accession Number

Q9NRN7

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEGVRWAFSC GTWLPSRAEW LLAVRSIQPE EKERIGQFVF ARDAKAAMAG RLMIRKLVAE KLNIPWNHIR LQRTAKGKPV LAKDSSNPYP NFNFNISHQG DYAVLAAEPE LQVGIDIMKT SFPGRGSIPE FFHIMKRKFT NKEWETIRSF KDEWTQLDMF YRNWALKESF IKAIGVGLGF ELQRLEFDLS PLNLDIGQVY KETRLFLDGE EEKEWAFEES KIDEHHFVAV ALRKPDGSRH QDVPSQDDSK PTQRQFTILN FNDLMSSAVP MTPEDPSFWD CFCFTEEIPI RNGTKS