Recombinant human CDA protein
CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.
Product Name Alternative
Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.
Molecular Weight
18.3 kDa (166aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Evrard A., et al. (2010) Ther Drug Monit. 32 (1) :53-60.; ; Seeberg EC., et a/. (1998) Blood. 91 (11) :4127-35.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100 mM NaCl, 40% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH54036.1
Uniprot Accession Number
P32320
Species
Human
AA Sequence
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