Recombinant human DCK protein

DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Deoxycytidine kinase, MGC117410, MGC138632, Deoxycytidine kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.6 kDa (296aa) confirmed by MALDI-TOF

Additionnal Information

DCK, ATGP0738-10 µg, ATGP0738-20 µg, ATGP0738-50 µg, ATGP0738-100 µg, ATGP0738-250 µg, ATGP0738-500 µg, ATGP0738-1 mg, ATGP0738-10, ATGP0738-20, ATGP0738-50, ATGP0738-100, ATGP0738-250, ATGP0738-500, ATGP0738-1

References & Citations

Radu CG., et al. (2010) Proc Natl Acad Sci u S A. 107 (12) :5551-6; ; Ribeiro R., et al. (2007) J Pharmacol Exp Ther. 323 (3) :935-45;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000779.1

Uniprot Accession Number

P27707

Species

Human

AA Sequence

MATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL