Recombinant human DCK protein
DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Deoxycytidine kinase, MGC117410, MGC138632, Deoxycytidine kinase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.6 kDa (296aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Radu CG., et al. (2010) Proc Natl Acad Sci u S A. 107 (12) :5551-6; ; Ribeiro R., et al. (2007) J Pharmacol Exp Ther. 323 (3) :935-45;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000779.1
Uniprot Accession Number
P27707
Species
Human
AA Sequence
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