Recombinant human ACOT13 protein
Acyl-coenzyme A thioesterase 13, also known ACOT13, belongs to the thioesterase subfamily of esterase family. Highly expressed in kidney with moderate expression in brain, liver and intestines, ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human ACOT13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Acyl-coenzyme A thioesterase 13, THEM2, HT012, PNAS-27, Acyl-coenzyme A thioesterase 13
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
17.1 kDa (160aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kanno K., et al. (2007) J Biol Chem. 282:30728-30736.; ; Cheng Z., et al. (2006) Biochem Biophys Res Commun. 350:850-853.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_060943
Uniprot Accession Number
Q9NPJ3
Species
Human
AA Sequence
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