Human Glutathione S-transferase mu 2/GSTM2 protein

GSTM2, also known as Glutathione S-transferase Mu 2, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase Mu 2, GST4, GSTM, GSTM2-2, GTHMuS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 250unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

27.9 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione S-transferase Mu2, GSTM2, Glutathione S-transferase Mu 2, GST4, GSTM, GSTM2-2, GTHMuS, Glutathione S-transferase Mu 2, ATGP0728-10 µg, ATGP0728-20 µg, ATGP0728-50 µg, ATGP0728-100 µg, ATGP0728-250 µg, ATGP0728-500 µg, ATGP0728-1 mg, ATGP0728-10, ATGP0728-20, ATGP0728-50, ATGP0728-100, ATGP0728-250, ATGP0728-500, ATGP0728-1

References & Citations

Hayes JD, et al. (1995) Crit Rev Biochem Mol Biol. 30 (6) :445-600. ; ; Bogaards JJ, et al. (1992) Biochem J. 286:383-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000839.1

Uniprot Accession Number

P28161

Species

Human

AA Sequence

MPMTLGYWNI RGLAHSIRLL LEYTDSSYEE KKYTMGDAPD YDRSQWLNEK FKLGLDFPNL PYLIDGTHKI TQSNAILRYI ARKHNLCGES EKEQIREDIL ENQFMDSRMQ LAKLCYDPDF EKLKPEYLQA LPEMLKLYSQ FLGKQPWFLG DKITFVDFIA YDVLERNQVF EPSCLDAFPN LKDFISRFEG LEKISAYMKS SRFLPRPVFT KMAVWGNK