Recombinant human HARS protein

HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

59.8 kDa (532aa) confirmed by MALDI-TOF

Additionnal Information

HARS, Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic, ATGP0726-10 µg, ATGP0726-20 µg, ATGP0726-50 µg, ATGP0726-100 µg, ATGP0726-250 µg, ATGP0726-500 µg, ATGP0726-1 mg, ATGP0726-10, ATGP0726-20, ATGP0726-50, ATGP0726-100, ATGP0726-250, ATGP0726-500, ATGP0726-1

References & Citations

Raben N., et al. (1992) Nucleic Acids Res. 20 (5) :1075-81. ; ; O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294 (3) :609-14.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002100

Uniprot Accession Number

P12081

Species

Human

AA Sequence

MAERAAL EELVKLQGER VRGLKQQKAS AELIEEEVAK LLKLKAQLGP DESKQKFVLK TPKGTRDYSP RQMAVREKVF DVIIRCFKRH GAEVIDTPVF ELKETLMGKY GEDSKLIYDL KDQGGELLSL RYDLTVPFAR YLAMNKLTNI KRYHIAKVYR RDNPAMTRGR YREFYQCDFD IAGNFDPMIP DAECLKIMCE ILSSLQIGDF LVKVNDRRIL DGMFAICGVS DSKFRTICSS VDKLDKVSWE EVKNEMVGEK GLAPEVADRI GDYVQQHGGV SLVEQLLQDP KLSQNKQALE GLGDLKLLFE YLTLFGIDDK ISFDLSLARG LDYYTGVIYE AVLLQTPAQA GEEPLGVGSV AAGGRYDGLV GMFDPKGRKV PCVGLSIGVE RIFSIVEQRL EALEEKIRTT ETQVLVASAQ KKLLEERLKL VSELWDAGIK AELLYKKNPK LLNQLQYCEE AGIPLVAIIG EQELKDGVIK LRSVTSREEV DVRREDLVEE IKRRTGQPLC IC