Recombinant human HARS protein
HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Product Specifications
Product Name Alternative
Histidyl-tRNA synthetase cytoplasmic, FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
59.8 kDa (532aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Raben N., et al. (1992) Nucleic Acids Res. 20 (5) :1075-81. ; ; O'Hanlon TP., et al. (2002) Biochem Biophys Res Commun. 294 (3) :609-14.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_002100
Uniprot Accession Number
P12081
Species
Human
AA Sequence
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