Recombinant human DUTPase/DUT protein

DuT, also known as duTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing duTP to duMP and diphosphate. This reaction is important for keeping the intracellular duTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DuT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DuT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

FLJ20622, duTPase, duTP pyrophosphatase, duTP diphosphatase, Deoxyuridine Triphosphatase, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.6 kDa (204aa) confirmed by MALDI-TOF

Additionnal Information

DuT, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, duTPase, duTP pyrophosphatase, FLJ20622, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, Deoxyuridine Triphosphatase, ATGP0725-10 µg, ATGP0725-20 µg, ATGP0725-50 µg, ATGP0725-100 µg, ATGP0725-250 µg, ATGP0725-500 µg, ATGP0725-1 mg, ATGP0725-10, ATGP0725-20, ATGP0725-50, ATGP0725-100, ATGP0725-250, ATGP0725-500, ATGP0725-1

References & Citations

Mclntosh EM., et al. (1992) Proc Natl Acad Sci u S A. 89 (17) :8020-4. ; ; Persson R., et al. (2001) Curr Protein Pept Sci. 2 (4) :287-300.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001020419

Uniprot Accession Number

P33316

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN