Recombinant human DIRAS1 protein

DIRAS1 belongs to a distinct branch of the functionally diverse Ras superfamily of monomeric GTPases. Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. Recombinant human DIRAS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GTP-binding protein Di-Ras1., Di-Ras1, FLJ42681, GBTS1, RIG, GTP-binding protein Di-Ras1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.1 kDa (215aa) confirmed by MALDI-TOF

Additionnal Information

DIRAS1, GTP-binding protein Di-Ras1., Di-Ras1, FLJ42681, GBTS1, RIG, GTP-binding protein Di-Ras1., ATGP0724-10 µg, ATGP0724-20 µg, ATGP0724-50 µg, ATGP0724-100 µg, ATGP0724-250 µg, ATGP0724-500 µg, ATGP0724-1 mg, ATGP0724-10, ATGP0724-20, ATGP0724-50, ATGP0724-100, ATGP0724-250, ATGP0724-500, ATGP0724-1

References & Citations

Ellis CA., et al. (2002) Proc Natl Acad Sci u S A. 99 (15) :9876-81. ; ; Kontani K., et al. (2002) J Biol Chem. 277 (43) :41070-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 1mM EDTA

Scientific Category

Cancer

NCBI Accession Number

NP_660156.1

Uniprot Accession Number

O95057

Species

Human

AA Sequence

MPEQSNDYRV VVFGAGGVGK SSLVLRFVKG TFRDTYIPTI EDTYRQVISC DKSVCTLQIT DTTGSHQFPA MQRLSISKGH AFILVFSVTS KQSLEELGPI YKLIVQIKGS VEDIPVMLVG NKCDETQREV DTREAQAVAQ EWKCAFMETS AKMNYNVKEL FQELLTLETR RNMSLNIDGK RSGKQKRTDR VKGKC