Recombinant human Secretagogin protein

SCGN, also known as secretagogin, is a cytoplasmic protein that contains six EF-hand domains and is related to the calicium-binding proteins Calretinin and Calbindin D28K. This protein is thought to be involved in cell proliferation and KCl (potassium chloride) -mediated calcium flux events. Through its interaction with KCl and its subsequent ability to modulate calcium storage pools within the cell, SCGN may function to negatively control growth and differentiation rates and, thus, indirectly inhibit cell replication. Recombinant SCGN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Secretagogin, CALBL, DJ501N12.8, SECRET, SEGN, setagin, Secretagogin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.2 kDa (296aa) confirmed by MALDI-TOF

Additionnal Information

Secretagogin, Secretagogin, CALBL, DJ501N12.8, SECRET, SEGN, setagin, Secretagogin, ATGP0722-10 µg, ATGP0722-20 µg, ATGP0722-50 µg, ATGP0722-100 µg, ATGP0722-250 µg, ATGP0722-500 µg, ATGP0722-1 mg, ATGP0722-10, ATGP0722-20, ATGP0722-50, ATGP0722-100, ATGP0722-250, ATGP0722-500, ATGP0722-1

References & Citations

Wagner L., et al. (2000) J Biol Chem. 275 (32) :24740-51. ; ; Gartner W., et al. (2001) Cereb Cortex. 11 (12) :1161-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Neuroscience

NCBI Accession Number

NP_008929

Uniprot Accession Number

O76038

Species

Human

AA Sequence

MDSSREPTLG RLDAAGFWQV WQRFDADEKG YIEEKELDAF FLHMLMKLGT DDTVMKANLH KVKQQFMTTQ DASKDGRIRM KELAGMFLSE DENFLLLFRR ENPLDSSVEF MQIWRKYDAD SSGFISAAEL RNFLRDLFLH HKKAISEAKL EEYTGTMMKI FDRNKDGRLD LNDLARILAL QENFLLQFKM DACSTEERKR DFEKIFAYYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILL RHCDVNKDGK IQKSELALCL GLKINP