Recombinant human Fibrillarin/FBL protein

FBL is a key small nucleolar protein in eukaryotes, which has an important role in pre-rRNA processing during ribosomal biogenesis. It is associated with the u3, u8, and u13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs) . Recombinant human FBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RRNA 2'-O-methyltransferase fibrillarin, FIB, FLRN, RNu3IP1, rRNA 2'-O-methyltransferase fibrillarin, Nop1, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.9 kDa (262aa), confirmed by MALDI-TOF

Additionnal Information

FBL, rRNA 2'-O-methyltransferase fibrillarin, FIB, FLRN, RNu3IP1, rRNA 2'-O-methyltransferase fibrillarin, ATGP0721-10 µg, ATGP0721-20 µg, ATGP0721-50 µg, ATGP0721-100 µg, ATGP0721-250 µg, ATGP0721-500 µg, ATGP0721-1 mg, ATGP0721-10, ATGP0721-20, ATGP0721-50, ATGP0721-100, ATGP0721-250, ATGP0721-500, ATGP0721-1

References & Citations

Amin MA, et al. (2007) Biochem Biophys Res Commun. 360 (2) :320-6; ; Jansen RP, et al. (1991) J Cell Biol 113 (4) : 715-29;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM EDTA

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001427

Uniprot Accession Number

P22087

Species

Human

AA Sequence

GKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN