Recombinant mouse LYPLA1 protein

LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity. Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.8 kDa (250aa) confirmed by MALDI-TOF

Additionnal Information

LYPLA1, Acyl-protein thioesterase 1, lysophospholipase 1, Pla1a, APT-1, LysoPLA I, LPL-I, APT1, ATGP0720-10 µg, ATGP0720-20 µg, ATGP0720-50 µg, ATGP0720-100 µg, ATGP0720-250 µg, ATGP0720-500 µg, ATGP0720-1 mg, ATGP0720-10, ATGP0720-20, ATGP0720-50, ATGP0720-100, ATGP0720-250, ATGP0720-500, ATGP0720-1

References & Citations

Schaloske R.H., et al. (2006) Biochim Biophys Acta. 1761:1246-1259.; ; Hiraoka M., et al. (2002) J. Biol. Chem 277:10090-10099.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_032892

Uniprot Accession Number

P97823

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID