Recombinant human 15-PGDH/HPGD protein

HPGD, also known as 15-PGDH, is the fundamental enzyme of prostaglandin degradation. This protein strongly reduces the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. HPGD is implicated in many physiologic and cellular processes, such as inflammation. Recombinant human HPGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

15-hydroxyprostaglandin dehydrogenase [NAD+], 15-PGDH, PGDH, PGDH1, SDR36C1, Prostaglandin dehydrogenase 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

31.1 (286aa) confirmed by MALDI-TOF

Additionnal Information

15 Hydroxyprostaglandin dehydrogenase, 15 Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-Hydroxyprostaglandin dehydrogenase, 15-Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, 15-PGDH, AV026552, HPGD, PGDH, PGDH 1, PGDH1, PHOAR 1, PHOAR1, Prostaglandin dehydrogenase 1, Prostaglandin dehydrogenase1, SDR36C 1, SDR36C1, ATGP0719-10 µg, ATGP0719-20 µg, ATGP0719-50 µg, ATGP0719-100 µg, ATGP0719-250 µg, ATGP0719-500 µg, ATGP0719-1 mg, ATGP0719-010, ATGP0719-020, ATGP0719-050, ATGP0719-100, ATGP0719-250, ATGP0719-500, ATGP0719-01M

References & Citations

Han X., et al. (1995) Equine Vet. J. 27: 334-339.; ; Van Meir C.A., et al. (1996) Placenta 17: 291-297.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_000851

Uniprot Accession Number

P15428

Species

Human

AA Sequence

MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KDMIKYYGIL DPPLIANGLI TLIEDDALNG AIMKITTSKG IHFQDYDTTP FQAKTQ