Recombinant human DDX39B protein

BAT1, also known as uAP56, is a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. This protein is an essential splicing factor required for association of u2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. Mutations in this protein may be associated with rheumatoid arthritis. Recombinant human BAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

DExD-box helicase 39B, HLA-B associated transcript 1, DEAD box polypeptide 39B, DEAD-box helicase 39B, U2AF65-associated protein 56, Spliceosome RNA helicase DDX39B, ATP-dependent RNA helicase p47, DEAD box protein UAP56, D6S81E, UAP56

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

51.1 (448aa) confirmed by MALDI-TOF

Additionnal Information

UAP56, U2AF65-associated protein 56, U2AF65 associated protein 56, Spliceosome RNA helicase DDX39B, Spliceosome RNA helicase BAT1, HLA-B associated transcript 1, HLA B associated transcript 1, DExD-box helicase 39B, DExD box helicase 39B, DEAD-box helicase 39B, DEAD box protein UAP56, DEAD box polypeptide 39B, DEAD box helicase 39B, DDX39B, D6S81E, BAT1, ATP-dependent RNA helicase p47, ATP dependent RNA helicase p47, ATGP0718-10 µg, ATGP0718-20 µg, ATGP0718-50 µg, ATGP0718-100 µg, ATGP0718-250 µg, ATGP0718-500 µg, ATGP0718-1 mg, ATGP0718-010, ATGP0718-020, ATGP0718-050, ATGP0718-100, ATGP0718-250, ATGP0718-500, ATGP0718-01M

References & Citations

Fleckner J., et al. (1997) Genes Dev. 11 (14) :1864-72.; ; Momose F., et al. (2001) J. Virol. 75 (4) :1899-908.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004631

Uniprot Accession Number

Q13838

Species

Human

AA Sequence

MAENDVDNEL LDYEDDEVET AAGGDGAEAP AKKDVKGSYV SIHSSGFRDF LLKPELLRAI VDCGFEHPSE VQHECIPQAI LGMDVLCQAK SGMGKTAVFV LATLQQLEPV TGQVSVLVMC HTRELAFQIS KEYERFSKYM PNVKVAVFFG GLSIKKDEEV LKKNCPHIVV GTPGRILALA RNKSLNLKHI KHFILDECDK MLEQLDMRRD VQEIFRMTPH EKQVMMFSAT LSKEIRPVCR KFMQDPMEIF VDDETKLTLH GLQQYYVKLK DNEKNRKLFD LLDVLEFNQV VIFVKSVQRC IALAQLLVEQ NFPAIAIHRG MPQEERLSRY QQFKDFQRRI LVATNLFGRG MDIERVNIAF NYDMPEDSDT YLHRVARAGR FGTKGLAITF VSDENDAKIL NDVQDRFEVN ISELPDEIDI SSYIEQTR