Recombinant human PKLR protein

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity > 80 units/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

Product Name Alternative

Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho (enol) pyruvate to pyruvate per minute at pH 7.5 at 37C.

Molecular Weight

59.2 kDa (549aa) confirmed by MALDI-TOF

Additionnal Information

PKLR, Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK, ATGP0714-10 µg, ATGP0714-20 µg, ATGP0714-50 µg, ATGP0714-100 µg, ATGP0714-250 µg, ATGP0714-500 µg, ATGP0714-1 mg, ATGP0714-10, ATGP0714-20, ATGP0714-50, ATGP0714-100, ATGP0714-250, ATGP0714-500, ATGP0714-1

References & Citations

Meza NW., et al. (2009) Mol Ther. 17 (12) :2000-9.; ; Roy MF., et al. (2007) J Exp Med. 204 (12) :2949-61.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_000289

Uniprot Accession Number

P30613

Species

Human

AA Sequence

MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS